Enzymatic Synthesis of Glycosaminoglycan Heparin

Enzymatic synthesis of glycosaminoglycan heparin.

Heparin and its low molecular weight heparin derivatives, widely used as clinical anticoagulants, are acidic polysaccharide members of a family of biomacromolecules called glycosaminoglycans (GAGs). Heparin and the related heparan sulfate are biosynthesized in the Golgi apparatus of eukaryotic cells. Heparin is a polycomponent drug that currently is prepared for clinical use by extraction from ...

Enzymatic synthesis of heparin related polysaccharides on sensor chips: rapid screening of heparin-protein interactions.

The biological roles of heparin (HP) and heparan sulfate (HS) are mediated mainly through their interaction with proteins. In the present work, we provide a rapid method for screening HP/HS-protein interactions providing structural data on the key sulfo groups that participate in the binding. A library of polysaccharides structurally related to HP was prepared by immobilizing the biotinylated N...

Regulation of Glycosaminoglycan Synthesis

0.1 ,uM triiodothyronine (T3) (1981. Endocrinology. 108: 2397). The current study characterizes that enhanced accumulation. Confluent cultures were maintained in thyroid hormnone-deficient media without or with added T3, labeled with [3H]acetate and analyzed for total [3H]GAG and [3H]hyaluronic acid content. Addition of T3 to thyroid hormone-depleted media consistently inhibited the incorporati...

Recent chemical and enzymatic approaches to the synthesis of glycosaminoglycan oligosaccharides.

Glycosaminoglycans, highly charged polycarboxylated, polysulfated polysaccharides, are an important class of therapeutic agents and investigational drug candidates. Heparin has been widely used as a clinical anticoagulant for over 60 years. Low molecular weight heparins have begun to displace heparin and recently a synthetic heparin pentasaccharide was approved for clinical use in Europe. In ad...

Knocking down glycosaminoglycan synthesis.